ABSTRACT
Two new, simple and cost effective UV-spectrophotometric and first order derivative methods were developed for estimation of tenofovir disoproxil fumarate in bulk and tablets. Tenofovir disoproxil fumarate was estimated at 260 nm in 0.1N HCl. In first order derivative, it showed amplitude at 273 nm. In both the methods linearity was found to be in the range of 5 - 40 micro g/ml; for UV-spectrophotometric method [Y = 0.02586 X+0.0083; r2 = 0.9999] and for first order derivative spectrophotometric method [Y = 0.00132 X+0.00035; r2 = 0.9995], respectively. These methods were tested and validated for various parameters according to USP guidelines. The quantitation limits were found to be 1.546 and 1.986 micro g/ml, for both the methods. The proposed methods were successfully applied for the determination of tenofovir disoproxil fumarate in pharmaceutical formulations. The results demonstrated that the procedure is accurate, precise and reproducible [relative standard deviation < 2%], while being simple, cheap and less time consuming and can be suitably applied for the estimation of tenofovir disoproxil fumarate in different dosage forms
Subject(s)
Fumarates/analysis , Spectrophotometry, Ultraviolet , Anti-HIV AgentsABSTRACT
Two analytical methods have been developed for simultaneous quantification of bisoprolol fumarate and hydrochlorothiazide in combined pharmaceutical dosage form using spectrophotometer. Excellent simplicity, accuracy, precision and economy were achieved by the assay. The 'method I' was based upon 'simultaneous equations' whereas the 'method II' was based upon 'multicomponent mode of analysis' of the instrument. For both these methods, 0.1 N NaOH was used as solvent. In this solvent, bisoprolol fumarate showed absorbance at 224 nm and hydrochlorothiazide at 273 nm. Linearity lies in the concentration range of 3 - 21 microg/ml for bisoprolol fumarate and 3 - 18 microg/ml for hydrochlorothiazide. The methods were validated statistically and by recovery studies